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当前位置:首页 > SouthernBiotech

SouthernBiotech(Southern?Biotechnology?Associates,?Inc)是由美国阿拉巴马大学伯明翰分校(UAB)Dr.?Max?Cooper细胞免疫学实验室中的科学家们于1982年创立,成立30年来一直致力于生产高质量、高亲和纯化的二抗、标记二抗以及其它免疫试剂,包括多种类型的多克隆和单克隆抗体、胶原蛋白和其它细胞外基质蛋白。


?多克隆二抗&试剂 ?细胞凋亡
?Anti:?Sheep,?Human,?Turkey,?Bovine,?Rhesus,?Chicken,?Porcine,?
?Hamster,?Canine,?Mouse,?Rat,?Equine,?Goat,?Rabbit,?Feline,?
?Guinea?Pig,?Streptavidin?and?Neutralite?Avidin
?ELISA?Sets,?ApoScreen?,?Recombinant?Proteins
?and Antibodies
?单克隆二抗 ?促炎症反应
?Anti:?Human,?Rhesus,?Chicken,?Hamster,?Mouse,?Rat,?Goat?and?Rabbit ?ELISA?Sets,?Recombinant?Proteins?and?Antibodies
?物种特异性二抗 ?免疫检测亚博体育app下载安卓
?Rhesus,?Mouse,?Goat?and?Rabbit ?Clonotyping/Isotyping,?Human?Cytokines,?Mouse?Cytokines
?and ?Apoptosis?Detection
?血清白蛋白抗体 ?纯化的胞外基质蛋白及抗体
?Bovine ?Collagen,?Laminin,?Antibodies,?Fibronectin
?CD分子和其他细胞表面蛋白抗体 ?细胞信号传导
?Anti:?Human,?Chicken,?Porcine,?Mouse,?Rabbit,?Feline ?Human,?Chicken,?Porcine,?Mouse,?Rat?and?Feline
?同型对照 ?酶底物
?Species:?Sheep,?Human,?Rhesus,?Chicken,?Donkey,?
?Porcine,?Hamster,?Canine,?Mouse,?Rat,?Goat?and?Rabbit
?病毒蛋白
?表观遗传&核信号传导 ?缓冲液
?Human?and?Mouse ?其他
?细胞因子 ?Mycoplasma?Detection?Kit,?Keratin?and?Anti-Biotin

SBA单抗分型多功能检测亚博体育app下载安卓?-----分型首选!
产品编号:
5300-04?(B)/?5300-05?(B)/?5300-01?(B)

在单克隆抗体的制备过程中,杂交瘤细胞的筛选和鉴别是最重要的步骤。首先需要筛选出正确融合的杂交瘤细胞,再筛选出能分泌目标抗体的杂交瘤细胞,最终将筛选出来的杂交瘤细胞进行扩大培养,制备大量单克隆抗体。在进行能分泌目标抗体杂交瘤细胞的筛选时,需要鉴定这类杂交瘤细胞培养上清液中单克隆抗体的类或亚类,最常用的是ELISA法,因其快速、简便、灵敏度高、特异性强、易于标准化等优点,已得到广泛应用。

实验原理


单克隆抗体分型ELISA亚博体育app下载安卓运用双抗体夹心法原理,先用Ig捕获二抗在96孔板上包被固相,然后加入待测的杂交瘤细胞培养上清液,培养上清液中的目标蛋白便会与96孔板上固相二抗结合,洗板去除游离物质,然后加入用于筛选杂交瘤细胞的标记二抗孵育,洗板去除游离物质,加入显色底物显色,在450nm处测定OD值,进行定性及定量分析。




亚博体育app下载安卓特点
●?操作流程简单,无需复杂的处理步骤,结果准确且易于分析;

●?多功能性,可用于初筛抗体高分泌杂交瘤细胞株,鉴定阳性克隆细胞的特异性抗体分型(IgA、IgM、IgG1、IgG2a、IgG2b、IgG3),?鉴定抗体kappa、lambda轻链异构体以及普通抗体分型ELISA研究;
●?性价比高,搭配使用可进行1000次以上样本检测;
●?严格的QC检测,确保批间一致性。

?


产品信息?(点击产品名称可连接到说明书)


?


?产品名称 ?产品编号 ?产品说明
Mouse?Immunoglobulin?Panel 5300-01 100ug*6种免疫球蛋白
B6/C57J?Mouse?Immunoglobulin?Panel 5300-01B 100ug*6种免疫球蛋白
SBA?Clonotyping?System-AP 5300-04 超过1000次HRP酶分析
SBA?Clonotyping?System-B6/C57J-AP 5300-04B 超过1000次HRP酶分析
SBA?Clonotyping?System-HRP 5300-05 超过1000次AP酶分析
SBA?Clonotyping?System-B6/C57J-HRP 5300-05B 超过1000次AP酶分析


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低内毒素无叠化合物二抗

内毒素是革兰氏阴性细菌细胞壁中的一种成分,也叫脂多糖,对宿主有毒性,当细菌死亡溶解或用人工方法破坏菌细胞后便释放出来,其毒性成分主要为类脂质A,耐热稳定,低内毒素是指产品含有非常微量的内毒素,是细胞和组织培养的理想选择。叠氮钠(Sodium?azide),白色粉末,分子式NaN3?,熔点275°C,沸点300°C,剧毒,和氰化物相似,对细胞色素氧化酶和其它酶有抑制作用,并能使体内氧合血红蛋白形成受阻,有显着的降压作用,对眼和皮肤有刺激性,如吸入、口服或经皮肤吸收,可引起中毒死亡,可用于血清防腐。无叠氮钠指产品中不含叠氮钠成分。

?产品名称 ?编号 ?抗体类型 ?宿主来源 ?规格
?Mouse?IgM-LE/AF ?0101-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgM-LE/AF ?0102-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgG2a-LE/AF ?0103-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgG2b-LE/AF ?0104-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgG3-LE/AF ?0105-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgA-LE/AF ?0106-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?IgG-LE/AF ?0107-14 ?Polyclonal ?Mouse ?0.5?mg
?Rat?IgG-LE/AF ?0108-14 ?Polyclonal ?Rat ?0.5?mg
?Mouse?F(ab’)2?IgG1-LE/AF ?0115-14 ?Monoclonal ?Mouse ?0.5?mg
?Rat?IgG1-LE/AF ?0116-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?IgG2a-LE/AF ?0117-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?IgG2b-LE/AF ?0118-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?IgM-LE/AF ?0120-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?IgG-LE/AF ?0121-14 ?Polyclonal ?Hamster ?0.5?mg
?Rat?Anti-Mouse?CD2/LFA-2-LE/AF ?1525-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?Anti-Mouse?CD3e-LE/AF ?1530-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Hamster?Anti-Mouse?CD3e-LE/AF ?1531-14 ?Monoclonal ?Hamster?(Syrian) ?0.5?mg
?Rat?Anti-Mouse?CD3e-LE/AF ?1535-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD8a/Ly-2-LE/AF ?1550-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD11b/Mac-1a-LE/AF ?1561-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD19-LE/AF ?1575-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD25/IL-2Ra-LE/AF ?1600-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?Anti-Mouse?CD28-LE/AF ?1610-14 ?Monoclonal ?Hamster?(Syrian) ?0.5?mg
?Hamster?Anti-Mouse?CD28-LE/AF ?1615-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Hamster?Anti-Mouse?CD30-LE/AF ?1620-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Rat?Anti-Mouse?CD38-LE/AF ?1635-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD38-LE/AF ?1640-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD40-LE/AF ?1645-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?Anti-Mouse?CD154/CD40L-LE/AF ?1650-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Rat?Anti-Mouse?CD54/ICAM-1-LE/AF ?1701-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD62L/L-selectin-LE/AF ?1705-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?Anti-Mouse?CD69/VEA-LE/AF ?1715-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Rat?Anti-Mouse?CD80/B7-1-LE/AF ?1730-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD86/B7-2-LE/AF ?1735-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD90/Thy-1-LE/AF ?1740-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD90.2/Thy-1.2-LE/AF ?1750-14 Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?Ly-6A/E-LE/AF ?1755-14 ?Monoclonal ?Rat ?0.5?mg
?Hamster?Anti-Mouse?TCRgd-LE/AF ?1780-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Hamster?Anti-Mouse?TCRb-LE/AF ?1785-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Hamster?Anti-Mouse?CD152/CTLA-4-LE/AF ?1790-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Hamster?Anti-Mouse?CD152/CTLA-4-LE/AF ?1791-14 ?Monoclonal ?Hamster?(Syrian) ?0.5?mg
?Mouse?Anti-Mouse?NK1.1-LE/AF ?1805-14 ?Monoclonal ?Mouse ?0.5?mg
?Hamster?Anti-Mouse?CD81-LE/AF ?1825-14 ?Monoclonal ?Hamster?(Armenian) ?0.5?mg
?Rat?Anti-Mouse?MAdCAM-1-LE/AF ?1865-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?LPAM-1-LE/AF ?1870-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD117/c-kit-LE/AF ?1880-14 ?Monoclonal ?Rat ?0.5?mg
?Mouse?Anti-Mouse?I-Ek-LE/AF ?1897-14 ?Monoclonal ?Mouse ?0.5?mg
?Rat?Anti-Mouse?Ly-6G/GR-1-LE/AF ?1900-14 ?Monoclonal ?Rat ?0.5?mg
?Mouse?Anti-Mouse?H-2Db-LE/AF ?1910-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Mouse?H-2Db-LE/AF ?1913-14 ?Monoclonal ?Mouse ?0.5?mg
?Rat?Anti-Mouse?CD86/B7-2-LE/AF ?1915-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?CD102/ICAM-2-LE/AF ?1925-14 ?Monoclonal ?Rat ?0.5?mg
?Mouse?F(ab’)2?Anti-Rat?IgM-LE/AF ?3082-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Human?CD9-LE/AF ?9310-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Human?CD11a/LFA-1a-LE/AF ?9320-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Human?CD3-LE/AF ?9515-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Human?CD95/Fas-LE/AF ?9730-14 ?Monoclonal ?Mouse ?0.5?mg
?Mouse?Anti-Human?CD95/Fas-LE/AF ?9735-14 ?Monoclonal ?Mouse ?0.5?mg
?Rat?Anti-Human?IL-10-LE/AF ?10100-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?GM-CSF-LE/AF ?10111-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?IL-3-LE/AF ?10115-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?IL-4-LE/AF ?10117-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?IL-7-LE/AF ?10122-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?IL-13-LE/AF ?10125-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Human?G-CSF-LE/AF ?10127-14 ?Monoclonal ?Rat ?0.5?mg
?Rat?Anti-Mouse?IL-4-LE/AF ?10203-14 上一品牌:Atlas antibodies 下一品牌:BioVision